Using Fluorescence Correlation Spectroscopy to Study Conformational Changes in Denatured Proteins

Fluorescence spectroscopy of conformational changes of single LH2 complexes.

We have investigated the energy landscape of the bacterial photosynthetic peripheral light-harvesting complex LH2 of purple bacterium Rhodopseudomonas acidophila by monitoring sequences of fluorescence spectra of single LH2 assemblies, at room temperature, with different excitation intensities as well as at elevated temperatures, utilizing a confocal microscope. The fluorescence peak wavelength...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Structural Changes in Polymer Gels Probed by Fluorescence Correlation Spectroscopy

Introduction Previous studies, e.g., by NMR,1,2 fluorescence pho­ tobleaching,3 and dynamic light scattering (DLS),4,5 have shown that the diffusion of noninteracting probe particles in polymer solutions and gels primarily de­ pends on the polymer concentration and the size of the probe. In interpreting these results, it is widely assumed that polymer gels behave like semidilute polymer solu­ t...

Fluorescence correlation spectroscopy.

Concepts in Fluorescence Correlation Spectroscopy

Fluorescence Correlation Spectroscopy (FCS) and related techniques are becoming widely used to measure molecular dynamics in a broad variety of specimens. These techniques provide important quantitative information on dynamics -such as diffusion coefficients, local concentration, binding reactions under equilibrium conditionswith high spatial and temporal resolutions. Also, they are minimal inv...